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Signagen/AAV-Syn-FLPo (AAV Serotype 1)/SL101447/>30 µL (Std Pack)
ProductCategory:Pre-maderecombinantAAV;AAV-Synapsin-FLPo;AAV-Syn-FLPo;AAV1-Synapsin-FLPo;AAV1-Syn-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheλintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV1-Synapsin-FLPopre-made rAAVinSEROtype1(withcapsidfromAAVserotype1and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderhumansynapsinpromoter(exclusiveneuronalexpression). Readytouseformat.
Titer:>1x1013VG/mL
Size:>30µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
VectorMap:
